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akt1 total monoclonal mouse  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc akt1 total monoclonal mouse
    Akt1 Total Monoclonal Mouse, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 2282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+akt1/Akt+(pan)+Mouse+mAb/pmc12705765-43-0-5
    Average 96 stars, based on 2282 article reviews
    akt1 total monoclonal mouse - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Produced:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).

    Sequencing:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).

    Western Blot:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).

    Isolation:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).

    TUNEL Assay:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).

    Staining:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).

    Incubation:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).

    Fluorescence:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).

    Expressing:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).

    Lysis:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).

    Sandwich ELISA:

    Article Title: β-Cell mass restoration by α7 nicotinic acetylcholine receptor activation
    Article Snippet: Membranes were incubated overnight at 4 °C with one of the following antibodies: total STAT3 (rabbit monoclonal, Cell Signaling Technology), phospho-STAT3–Tyr 705 (rabbit monoclonal, Cell Signaling), phospho-NF-κB–Ser 536 (rabbit monoclonal, Cell Signaling), iNOS/NOS2 (rabbit polyclonal, Millipore), IRS-2 (rabbit polyclonal, Cell Signaling Technology), phospho-Akt–Ser 473 (rabbit monoclonal, Cell Signaling Technology), total Akt1 (rabbit monoclonal, Cell Signaling Technology), total CREB (rabbit polyclonal, Cell Signaling Technology), phospho-CREB–Ser 133 , and Pdx1 (rabbit polyclonal, Millipore).

    Article Title: Mechanism of action of the trastuzumab biosimilar CT-P6.
    Article Snippet: Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments.. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2overexpressing breast cancer (in both the adjuvant and metastatic settings) and HER2-positive gastric cancer.. CT-P6 (Herzuma®) is a biosimilar of trastuzumab, designed to bind with high affinity and specificity to the same HER2 epitope as the reference product.

    Article Title: Iron induces insulin resistance in cardiomyocytes via regulation of oxidative stress
    Article Snippet: The following primary antibodies were used in this study: pAkt T308 (4026), Total Akt1 (9272), LC3B (2775), P62 (23214), Beclin-1 (3738), pmTORC1 S2448 (2971), pULK1 S757 (14202), pAMPK T172 (2535), p70S6K T389 (9234) and GAPDH (2118) were purchased from Cell Signalling Technology. pIR beta Y972 and pIRS-1 Y612 (44816) were purchased from Life Technology.

    Article Title: Understanding the function of Pax5 in development of docetaxel-resistant neuroendocrine-like prostate cancers
    Article Snippet: Total Akt1 , 1:1000 , Cell Signaling, 2967S.

    Article Title: Musclin peptides and methods of use thereof
    Article Snippet: Western blotting: Whole cell protein extracts were prepared by homogenizing skeletal muscle tissue in NaCl 150 mM, Tris-HCl 50 mM (pH 7.8), supplemented with 1.5% Triton X-100, protease and phosphatase inhibitors (Roche).

    Article Title: Overexpression of MyrAkt1 in Endothelial Cells Leads to Erythropoietin- and BMP4-Independent Splenic Erythropoiesis in Mice
    Article Snippet: Protein levels were assessed by Western blot with antibodies against phospho-Akt1 (Ser473) (9271, Cell Signaling Technology, Danvers, MA), total Akt1 (9272, Cell Signaling Technology), and GAPDH (374, Millipore, Billerica, MA).



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    Image Search Results


    Autophagy and mTOR/AKT pathway in AS neuronal cells’ model. ( A – D ) Cell lysates from control and UBE3A − SH-SY5Y cells were used for western blotting analysis using specific antibodies to monitor autophagy activation, i.e., p62 (panel A ) and LC3b (I and II, panel C ). A ‘stain-free protein normalization’ method was used for the normalization of bands to total protein in blots, eliminating the need for housekeeping proteins. The data are expressed as % vs. control cells (for p62) and as ratio LC3b II/LC3b I, and represent the mean ± SEM of four independent experiments. Comparisons between control and UB3A − were performed using an unpaired two-tailed Student’s t -test; *** p < 0.001. ( E , F ) SH-SY5Y (control and UBE3A − cells) were fixed, and specific antibodies (total mTOR and phospho-mTOR, (panel E ); total AKT or phospho-AKT, (panel F )) were used in specific immunoezymatic assays, as reported in . The data are expressed as the ratio between phosphorylated and total protein levels (in % vs. control cells) and represent the mean ± SEM of at four independent experiments. Comparisons between control and UBE3A − were performed using an unpaired two-tailed Student’s t -test; *** p < 0.001 vs. control cells.

    Journal: International Journal of Molecular Sciences

    Article Title: Dysfunction of the Autophagy System and MDM2–p53 Axis Leads to the Accumulation of Amyloidogenic Proteins in Angelman Syndrome Models

    doi: 10.3390/ijms262211032

    Figure Lengend Snippet: Autophagy and mTOR/AKT pathway in AS neuronal cells’ model. ( A – D ) Cell lysates from control and UBE3A − SH-SY5Y cells were used for western blotting analysis using specific antibodies to monitor autophagy activation, i.e., p62 (panel A ) and LC3b (I and II, panel C ). A ‘stain-free protein normalization’ method was used for the normalization of bands to total protein in blots, eliminating the need for housekeeping proteins. The data are expressed as % vs. control cells (for p62) and as ratio LC3b II/LC3b I, and represent the mean ± SEM of four independent experiments. Comparisons between control and UB3A − were performed using an unpaired two-tailed Student’s t -test; *** p < 0.001. ( E , F ) SH-SY5Y (control and UBE3A − cells) were fixed, and specific antibodies (total mTOR and phospho-mTOR, (panel E ); total AKT or phospho-AKT, (panel F )) were used in specific immunoezymatic assays, as reported in . The data are expressed as the ratio between phosphorylated and total protein levels (in % vs. control cells) and represent the mean ± SEM of at four independent experiments. Comparisons between control and UBE3A − were performed using an unpaired two-tailed Student’s t -test; *** p < 0.001 vs. control cells.

    Article Snippet: The primary antibodies (total AKT, Antibody, sc81434; phospho-AKT (Ser473), sc7985, both Santa Cruz Biotechnology, Santa Cruz, CA, USA; mTOR Antibody #2972; Phospho-mTOR (Ser2448) Antibody #2971, both Cell Signaling Technology, USA) were diluted at 1:300 in a 1% BSA-0.03% Triton X-100 solution at 4 °C overnight.

    Techniques: Control, Western Blot, Activation Assay, Staining, Two Tailed Test